|Title:||A NMR reverse diffusion filter for the simplification of spectra of complex mixtures and the study of drug receptor interactions|
|Authors:||M. Vega-Vázquez, J. C. Cobas, F. F. Oliveira de Sousa, M. Martin-Pastor M. Vega-Vázquez, J. C. Cobas, F. F. Oliveira de Sousa, M. Martin-Pastor|
|Reference:||Magn. Res. Chem. Volume 49, Issue 8, pages 464–468, August 2011 Magnetic Resonance in Chemistry Volume 49, Issue 8, pages 464–468, August 2011|
A reverse diffusion filter NMR experiment (Drev) is proposed for the study of small molecules in binding with macromolecules. The filtering efficiency of Drev to eliminate the signals of the macromolecule is shown to be superior to conventional transverse relaxation filters at least for macromolecules containing a significant fraction of flexible residues. The Drev filter was also a useful complement for ligand-based NMR screening in combination with saturation transfer difference experiments.