Fermentanomics: Monitoring Mammalian Cell Cultures with NMR Spectroscopy

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Title: Fermentanomics: Monitoring Mammalian Cell Cultures with NMR Spectroscopy
Authors: Scott A. Bradley , Anli Ouyang , Jennifer Purdie , Tim A. Smitka , Tongtong Wang and Andreas Kaerner. Several Researchers at Lilly
Date: 2010/08/03
Reference: J. Am. Chem. Soc., 2010, 132 (28), pp 9531–9533
DOI: http://dx.doi.org/10.1021/ja101962c

 

Scientists at Lilly have now established the basis of a new –omics related discipline; which they have dubbed Fermentanomics and consists in a new rapid and robust NMR method for monitoring mammalian cell cultures.

 

As the number of therapeutic proteins produced by mammalian cell cultures in the pharmaceutical industry continues to increase, the need to improve productivity and ensure consistent product quality during process development activities becomes more significant. Rational medium design is known to improve cell culture performance, but an understanding of nutrient consumption and metabolite accumulation within the medium is required. To this end, we have developed a technique for using 1D (1)H NMR to quantitate nonprotein feed components and metabolites in mammalian cell cultures.

We refer to the methodology as “Fermentanomics” to differentiate it from standard metabolomics. The method was found to generate spectra with excellent water suppression, signal-to-noise, and resolution. More importantly, nutrient consumption and metabolite accumulation was readily observed. In total, 50 media components have been identified and quantitated.

The application of Fermentanomics to the optimization of a propietary CHO basal medium yielded valuable insight regarding the nutrient levels needed to maintain productivity. While the focus here is on the extracellular milieu of CHO cell cultures, this methodology is generally applicable to quantitating intracellular concentrations and can be extended to other mammalian cell lines, as well as platforms such as yeasts, fungi, and Escherichia coli.

Scientists at Lilly have now established the basis of a new –omics related discipline which they have dubbed Fermentanomics and consists in a new rapid and robust NMR method for monitoring mammalian cell cultures. They have used Global Spectral Deconvolution (GSD) technique available in Mnova for the extraction of the concentrations of the components from the NMR spectra of the spent media of mammalian cell culture. Find more at the Carlos NMR Analysis and Processing Blog or at their JACS article.

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